A resting cell system for efrotomycin biosynthesis.
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منابع مشابه
A resting cell system for efrotomycin biosynthesis.
Efrotomycin, a modified polyketide antibiotic with utility as a growth permittant in the animal industry, is produced by Nocardia lactamdurans. A resting cell system has been developed to facilitate biosynthetic studies. Washed cells harvested from oil-based medium at 64 hours and resuspended in buffer produce up to 700 mg/ml efrotomycin in 60 hours when supplemented with a carbon source, optim...
متن کاملThe structure of efrotomycin.
The antibiotic efrotomycin (I), C59H88N2O20, was isolated from cultures of Nocardia lactamdurans as an amorphous yellow powder. Mass spectral and NMR analyses show that the compound is a glycoside of the known antibiotic aurodox (II), C44H62N2O12. Ozonolysis and hydrolysis of I produced the disaccharide V, 6-deoxy-4-O-(6-deoxy-2,4-di-O-methyl-alpha -L-mannopyranosyl)-3-O-methyl-beta-D-allopyran...
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BETTINA M. FROST, MARY E. VALIANT and EUGENE L. DULANEY Merck Institute for Therapeutic Research Rahway, New Jersey, 07065, U.S.A. (Received for publication May 26, 1979) Efrotomycin1) is a narrow spectrum antibiotic which is most active in vitro against representative species of Pasteurella, Moraxella and Corynebacterium2) . The in vivo activity, however, is much better than one would expect f...
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A crude homogenate obtained from greening cucumber (Cucumis sativus, L.) cotyledons in tris-sucrose, pH 7.7, containing coenzyme A, glutathione, potassium phosphate, pH 7.7, methyl alcohol, magnesium, nicotinamide adenine dinucleotide, and adenosine triphosphate, is able to incorporate 4-(14)C-delta-aminolevulinic acid into chlorophyll a and b in the presence of oxygen. If the homogenates are p...
متن کاملThe biosynthesis of ferredoxin in a cell-free system.
The investigation reported in this communication was concerned with the biosynthesis of ferredoxin in a cell-free system. A cell-free system, with the ability to incorporate amino acids into peptides and proteins, was developed from Cl. pasteurianum, and its requirements were established. After the incorporation of L-alanine-C(14), ferredoxin-C(14) was isolated in crystalline form with the aid ...
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ژورنال
عنوان ژورنال: The Journal of Antibiotics
سال: 1989
ISSN: 0021-8820,1881-1469
DOI: 10.7164/antibiotics.42.944